Vacuum fills the evacuated collection tubes with a predetermined volume of blood. Availability of different sizes of tubes makes them easy to use for pediatric and small volume collections. The tubes can have different additives or coatings so the mixing of blood from one tube to another should not be done. When blood is collected, it mixes with the different additives present in the tubes Color coding of the plastic caps specifies the additives contained in that test tube.
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Tube tops are color coded to indicate the additive present.